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Product Description
GAPDH Monoclonal Antibody | E-AB-20032 | Elabscience
Type: Control Antibody
Synonyms: Aging associated gene 9 protein, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, Peptidyl-cysteine S-nitrosylase GAPDH
Application: WB, IHC-p, IF
Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Hamster, Pig, Rabbit, Sheep, Insect, Yeast
Host: Mouse
Isotype: IgG
Reserch Areas: Cancer, Metabolism, Signal Transduction
Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Synthetic Peptide
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method: Protein A purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Clone: Clone:9S5
Conjugation: Unconjugated
Molecular Weight(Calculated): N/A
Molecular Weight(Observed): 37 kDa
