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Product Description
MBP-Tag Monoclonal Antibody | E-AB-20013 | Elabscience
Type: Tag Antibody
Synonyms: maltose-binding protein, MBP
Application: WB
Reactivity: N/A
Host: Mouse
Isotype: IgG
Reserch Areas: Tags and Cell Markers
Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
Concentration: 1 mg/mL
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Immunogen: Recombinant Protein
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method: Protein A purification
Dilution: WB 1:5000-1:10000
Clone: Clone:8K2
Conjugation: Unconjugated
Molecular Weight(Calculated): N/A
Molecular Weight(Observed): N/A
