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Product Description
Rat Renal Fibroblast | EP-CP-R069 | Elabscience
Background: Rat kidney fibroblasts are isolated from kidney Tissue. The kidney is an important organ of the body. Its basic function is to produce urine to remove metabolites and certain wastes and poisons from the body. At the same time, it retains water and other usefulness substances (such as glucose, protein, amino acids, sodium ions, potassium ions, sodium bicarbonate, etc.) through reabsorption to regulate water, electrolyte balance and maintain acid-base balance. The kidney also has endocrine functions, producing renin, erythropoietin, active vitamin D3, prostaglandin, kinin, etc. It is also the degradation site of part of the body's endocrine hormones and the target organ of extrarenal hormones. These functions of the kidneys ensure the stability of the body's environment and allow the metabolism to proceed normally. Fibroblasts are the main cellular components of loose connective Tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and Tissue defects Effect. The newly isolated kidney fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. Its main functions are: 1. constitute an important part of the inner wall of the filter barrier. 2. synthesize necessary bioactive molecules under the stimulation of inflammation and thrombosis. 3. affect mesangial cells and epithelial cells after damage, and then affect kidney disease. The rat kidney fibroblasts produced by our company are prepared by trypsin-collagenase mixed digestion method combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by Vimentin immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.
Renewal: Every 2-3 days
Ratio: 1:2-1:3
Medium: EP-MP-R069
Growth Properties: Adherent
Cell Type: Fibroblast
Tissue Type: Urinary system
Tissue: Kidney Tissue
Organism: Rat
