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Product Description
Human CK-19/KRT19 (Cytokeratin 19) ELISA Kit | E-EL-H2075 | Elabscience
Type: Sandwich
Assay Time: 3.5h
Sensitivity: 37.5pg/mL
Detection Range: 62.5~4000pg/mL
UniProt ID: P08727
Target Name: CK-19/KRT19
Target Synonym: CK19, K19, Keratin 19, K1CS, Keratin, type I cytoskeletal 19, 40-kDa Keratin Intermediate Filament
Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CK-19/KRT19. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CK-19/KRT19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CK-19/KRT19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CK-19/KRT19. You can calculate the concentration of Human CK-19/KRT19 in the samples by comparing the OD of the samples to the standard curve.
Specifivity: This kit recognizes Human CK-19/KRT19 in samples. No significant cross-reactivity or interference between Human CK-19/KRT19 and analogues was observed.
Research Areas: Cancer, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
