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Product Description
Cattle HGF (Hepatocyte Growth Factor) ELISA Kit | ELK6687 | ELK Biotechnology
Alternative Names: HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
Species: Cattle
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor (HGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor (HGF) in the samples is then determined by comparing the OD of the samples to the standard curve.
