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Product Description
Cattle TGFb1 (Transforming Growth Factor Beta 1) ELISA Kit | ELK5836 | ELK Biotechnology
Alternative Names: TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Species: Cattle
Assay Type: Sandwich
Sensitivity: 10.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the OD of the samples to the standard curve.
