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Product Description
Chicken IL16 (Interleukin 16) ELISA Kit | ELK2601 | ELK Biotechnology
Alternative Names: IL-16;LCF; PrIL-16; Lymphocyte Chemoattractant Factor; Pro-Interleukin 16
Species: Chicken
Assay Type: Sandwich
Sensitivity: 3.3 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Cytokine;Infection immunity;Autoimmunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 16 (IL16) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 16 (IL16) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 16 (IL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 16 (IL16) in the samples is then determined by comparing the OD of the samples to the standard curve.
