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Product Description
Canine D2D (D-Dimer) ELISA Kit | ELK9371 | ELK Biotechnology
Alternative Names: D 2 Dimer
Species: Canine
Assay Type: Competitive Inhibition
Sensitivity: 27.2 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: Plasma
Assay Lenght: 2h
Research Area: Hematology;Cardiovascular biology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with D-Dimer (D2D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to D-Dimer (D2D) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Dimer (D2D) in the samples is then determined by comparing the OD of the samples to the standard curve.
