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Product Description
General 1, 5-AG (1, 5-Anhydroglucitol) ELISA Kit | ELK7840 | ELK Biotechnology
Alternative Names: LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1, 5-Anhydro-D-glucitol; 1, 5-Anhydrosorbitol; Aceritol; Polygalytol
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.6 μg/mL
Standard: 150 μg/mL
Detection Range: 2.35-150 μg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1, 5-Anhydroglucitol (1, 5-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1, 5-Anhydroglucitol (1, 5-AG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1, 5-Anhydroglucitol (1, 5-AG) in the samples is then determined by comparing the OD of the samples to the standard curve.
