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Product Description
General 4-HNE (4-Hydroxynonenal) ELISA Kit | ELK9581 | ELK Biotechnology
Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.38 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-Hydroxynonenal (4-HNE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxynonenal (4-HNE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxynonenal (4-HNE) in the samples is then determined by comparing the OD of the samples to the standard curve.
