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Product Description
General 5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8155 | ELK Biotechnology
Alternative Names: 5-HETE
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 3.95 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Enzyme & Kinase
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyeicosatetraenoic Acid (5-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.
