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Product Description
General CML (Carboxymethyl Lysine) ELISA Kit | ELK7896 | ELK Biotechnology
Alternative Names: N (6) -Carboxymethyllysine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 25.9 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Genetic science;Nutrition metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carboxymethyl Lysine (CML) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carboxymethyl Lysine (CML) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxymethyl Lysine (CML) in the samples is then determined by comparing the OD of the samples to the standard curve.
