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Product Description
General ET (Epitestosterone) ELISA Kit | ELK7878 | ELK Biotechnology
Alternative Names: ET
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 15.29 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Epitestosterone (ET) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Epitestosterone (ET) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epitestosterone (ET) in the samples is then determined by comparing the OD of the samples to the standard curve.
