Product Description
General GA (Gibberellic Acid) ELISA Kit | ELK7853 | ELK Biotechnology
Alternative Names: GA3; Gibberellin A3; Gibberellins
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 52.1 ng/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 ng/mL
Sample Type: tissue or cell culture supernates
Assay Lenght: 2h
Research Area: Nutrition metabolism;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gibberellic Acid (GA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gibberellic Acid (GA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gibberellic Acid (GA) in the samples is then determined by comparing the OD of the samples to the standard curve.