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Product Description
General HVD3 (25-Hydroxyvitamin D3) ELISA Kit | ELK7890 | ELK Biotechnology
Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH) D
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3 (HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3 (HVD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3 (HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.
