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Product Description
General IAA (Indole 3 Acetic Acid) ELISA Kit | ELK7852 | ELK Biotechnology
Alternative Names: IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.89 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: tissue or cell culture supernates
Assay Lenght: 2h
Research Area: Signal transduction;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Indole 3 Acetic Acid (IAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indole 3 Acetic Acid (IAA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indole 3 Acetic Acid (IAA) in the samples is then determined by comparing the OD of the samples to the standard curve.
