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Product Description
General PEG (Polyethylene Glycol) ELISA Kit | ELK8264 | ELK Biotechnology
Alternative Names: PEO; POE; Polyethylene oxide; Polyoxyethylene; Carbowax; GoLYTELY; GlycoLax; Fortrans; TriLyte; Colyte; Halflytely; Macrogol; MiraLAX; MoviPrep
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 4.72 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: biological agents
Assay Lenght: 2h
Research Area: Gastrointestinal science
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Polyethylene Glycol (PEG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polyethylene Glycol (PEG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polyethylene Glycol (PEG) in the samples is then determined by comparing the OD of the samples to the standard curve.
