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Product Description
General rT3 (Reverse Triiodothyronine) ELISA Kit | ELK7898 | ELK Biotechnology
Alternative Names: RT3; r-T3; Reverse T3; (2S) -2-Amino-3-[4- (4-Hydroxy-3, 5-Diiodophenoxy) -3-Iodophenyl]propanoic Acid; 3, 3', 5'-Triiodo-L-thyronine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 27.55 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine (rT3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Reverse Triiodothyronine (rT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reverse Triiodothyronine (rT3) in the samples is then determined by comparing the OD of the samples to the standard curve.
