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Product Description
General RVT (Resveratrol) ELISA Kit | ELK8265 | ELK Biotechnology
Alternative Names: trans-3, 5, 4'-Trihydroxystilbene; 3, 4', 5-Stilbenetriol; trans-Resveratrol
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 89.3 ng/mL
Standard: 20000 ng/mL
Detection Range: 312.5-20000 ng/mL
Sample Type: plant tissue and biological fluids
Assay Lenght: 2h
Research Area: Cardiocerebral vascular system
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Resveratrol (RVT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Resveratrol (RVT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resveratrol (RVT) in the samples is then determined by comparing the OD of the samples to the standard curve.
