Euro
USD
British Pound
NULL
Product Description
General SP (Substance P) ELISA KitKit | ELK9577 | ELK Biotechnology
Alternative Names: TAC1;TAC2;NKNA
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 46.88 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology;Neuro science;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Substance PKit (SP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Substance PKit (SP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Substance PKit (SP) in the samples is then determined by comparing the OD of the samples to the standard curve.
