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Product Description
General VD3 (Vitamin D3) ELISA Kit | ELK8102 | ELK Biotechnology
Alternative Names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 2.14 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin D3 (VD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D3 (VD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D3 (VD3) in the samples is then determined by comparing the OD of the samples to the standard curve.
