Euro
USD
British Pound
NULL
Product Description
General VMA (Vanillylmandelic Acid) ELISA Kit | ELK8239 | ELK Biotechnology
Alternative Names: Vanillyl Mandelic Acid
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 43.7 ng/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 ng/mL
Sample Type: urine and biological agents
Assay Lenght: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vanillylmandelic Acid (VMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vanillylmandelic Acid (VMA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vanillylmandelic Acid (VMA) in the samples is then determined by comparing the OD of the samples to the standard curve.
