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Product Description
Plant CYT (Cytokinin) ELISA Kit | ELK8628 | ELK Biotechnology
Alternative Names: CYT
Species: Plant
Assay Type: Competitive Inhibition
Sensitivity: 0.062 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: plant tissues
Assay Lenght: 2h
Research Area: Enzyme & Kinase
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cytokinin (CYT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytokinin (CYT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokinin (CYT) in the samples is then determined by comparing the OD of the samples to the standard curve.
