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Product Description
Dog ANGPT1 (Angiopoietin 1) ELISA Kit | ELK6506 | ELK Biotechnology
Alternative Names: ANG1; AGP1; AGPT; ANG-I
Species: Dog
Assay Type: Sandwich
Sensitivity: 0.93 ng/mL
Standard: 150 ng/mL
Detection Range: 2.35-150 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Cardiovascular biology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 1 (ANGPT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiopoietin 1 (ANGPT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin 1 (ANGPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin 1 (ANGPT1) in the samples is then determined by comparing the OD of the samples to the standard curve.
