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Product Description
Dog KAL (Kallistatin) ELISA Kit | ELK8911 | ELK Biotechnology
Alternative Names: SERPINA4; KLST; KST; PI4; Serpin Peptidase Inhibitor Clade A Member 4; Tissue Kallikrein Inhibitor; Kallikrein Binding Protein; Peptidase inhibitor 4
Species: Dog
Assay Type: Sandwich
Sensitivity: 2.46 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallistatin (KAL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallistatin (KAL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallistatin (KAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallistatin (KAL) in the samples is then determined by comparing the OD of the samples to the standard curve.
