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Product Description
Dog SAA (Serum Amyloid A) ELISA Kit | ELK8932 | ELK Biotechnology
Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
Species: Dog
Assay Type: Sandwich
Sensitivity: 0.37 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Infection immunity;Kidney biomarker;Hepatology;Urology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A (SAA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serum Amyloid A (SAA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid A (SAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A (SAA) in the samples is then determined by comparing the OD of the samples to the standard curve.
