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Product Description
Horse COL3 (Collagen Type III) ELISA Kit | ELK8221 | ELK Biotechnology
Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III
Species: Horse
Assay Type: Competitive Inhibition
Sensitivity: 4.98 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Cardiovascular biology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Collagen Type III (COL3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type III (COL3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type III (COL3) in the samples is then determined by comparing the OD of the samples to the standard curve.
