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Product Description
Human 15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit | ELK8351 | ELK Biotechnology
Alternative Names: 15-HETE; 15-Hydroxyeicosatetraenoic Acid)
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.1 ng/mL
Standard: 8 ng/mL
Detection Range: 0.13-8 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 15-Hydroxyeicosatetraenoic Acid (15-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 15-Hydroxyeicosatetraenoic Acid (15-HETE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 15-Hydroxyeicosatetraenoic Acid (15-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.
