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Product Description
Human 8-OHdG (DNA Damage) ELISA Kit | ELK8533 | ELK Biotechnology
Alternative Names: 8OHdG; 7, 8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7, 8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DNA Damage (8-OHdG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DNA Damage (8-OHdG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Damage (8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve.
