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Product Description
Human ADCYAP1 (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit | ELK4860 | ELK Biotechnology
Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.55 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
