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Product Description
Human aEP (Alpha-Endorphin) ELISA Kit | ELK2087 | ELK Biotechnology
Alternative Names: AEP
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 4.45 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluid.
Assay Lenght: 2h
Research Area: Signal transduction;Endocrinology;Neuro science;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Endorphin (aEP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Endorphin (aEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Endorphin (aEP) in the samples is then determined by comparing the OD of the samples to the standard curve.
