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Product Description
Human CPLX2 (Complexin 2) ELISA Kit | ELK5608 | ELK Biotechnology
Alternative Names: 921-L; CPX-2; CPX2; Synaphin-1
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 86.3 pg/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 2h
Research Area: Immune molecule
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Complexin 2 (CPLX2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complexin 2 (CPLX2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complexin 2 (CPLX2) in the samples is then determined by comparing the OD of the samples to the standard curve.
