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Product Description
Human KLK8 (Kallikrein 8) ELISA Kit | ELK1851 | ELK Biotechnology
Alternative Names: NP; HNP; NRPN; PRSS19; TADG14; BSP1; Neuropsin; Ovasin; Brain Serine Protease 1; Tumor Associated Differentially Expressed Gene 14; Serine protease 19
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 225.3 pg/mL
Standard: 50000 pg/mL
Detection Range: 781.25-50000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 2h
Research Area: Enzyme & Kinase;Tumor immunity;Neuro science
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 8 (KLK8) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 8 (KLK8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 8 (KLK8) in the samples is then determined by comparing the OD of the samples to the standard curve.
