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Product Description
Human Tb4 (Thymosin Beta 4) ELISA Kit | ELK4391 | ELK Biotechnology
Alternative Names: TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin, Beta 4, X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Endocrinology;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 4 (Tb4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 4 (Tb4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymosin Beta 4 (Tb4) in the samples is then determined by comparing the OD of the samples to the standard curve.
