Euro
USD
British Pound
NULL
Product Description
Human UCN3 (Urocortin 3) ELISA Kit | ELK5061 | ELK Biotechnology
Alternative Names: SCP; SPC; UCNIII; Stresscopin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 9.81 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 2h
Research Area: Endocrinology
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Urocortin 3 (UCN3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urocortin 3 (UCN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urocortin 3 (UCN3) in the samples is then determined by comparing the OD of the samples to the standard curve.
