Euro
USD
British Pound
NULL
Product Description
Human AANAT (Arylalkylamine-N-Acetyltransferase) ELISA Kit | ELK5237 | ELK Biotechnology
Alternative Names: AA-NAT; SNAT; Aralkylamine N-Acetyltransferase; Serotonin Acetyltransferase; Serotonin Acetylase; Serotonin N-Acetyltransferase; Melatonin Rhythm Enzyme
Species: Human
Assay Type: Sandwich
Sensitivity: 0.35 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;Developmental science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylalkylamine-N-Acetyltransferase (AANAT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Arylalkylamine-N-Acetyltransferase (AANAT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylalkylamine-N-Acetyltransferase (AANAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylalkylamine-N-Acetyltransferase (AANAT) in the samples is then determined by comparing the OD of the samples to the standard curve.
