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Product Description
Human ADPRH (ADP Ribosylarginine Hydrolase) ELISA Kit | ELK5362 | ELK Biotechnology
Alternative Names: ARH1; ADP-ribose-L-arginine cleaving enzyme; [Protein ADP-ribosylarginine] hydrolase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosylarginine Hydrolase (ADPRH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADP Ribosylarginine Hydrolase (ADPRH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosylarginine Hydrolase (ADPRH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP Ribosylarginine Hydrolase (ADPRH) in the samples is then determined by comparing the OD of the samples to the standard curve.
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![Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/388607/548758/sab-signalway-antibody__92073.1641690747__91737.1641697878__93467.1641864662__88276.1642545351__28504.1642558555.gif?c=2 "Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit")
![Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/847897/1139484/abebio__10018.1658410391__37521.1658420002.png?c=2 "Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit")
