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Product Description
Human CLEC3B (C-Type Lectin Domain Family 3, Member B) ELISA Kit | ELK6087 | ELK Biotechnology
Alternative Names: TN; TNA; Tetranectin; Plasminogen Binding Protein; Plasminogen kringle 4-binding protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.06 ng/mL
Standard: 12 ng/mL
Detection Range: 0.19-12 ng/mL
Sample Type: Plasma.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Lectin Domain Family 3, Member B (CLEC3B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to C-Type Lectin Domain Family 3, Member B (CLEC3B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Lectin Domain Family 3, Member B (CLEC3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Lectin Domain Family 3, Member B (CLEC3B) in the samples is then determined by comparing the OD of the samples to the standard curve.
