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Product Description
Human CYP2C9 (Cytochrome P450 2C9) ELISA Kit | ELK7438 | ELK Biotechnology
Alternative Names: CYP2C10; P450IIC9; Cytochrome P450, Subfamily IIC (Mephenytoin 4-Hydroxylase) Polypeptide 9; S-mephenytoin 4-hydroxylase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.236 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C9 (CYP2C9) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 2C9 (CYP2C9) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C9 (CYP2C9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2C9 (CYP2C9) in the samples is then determined by comparing the OD of the samples to the standard curve.
