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Product Description
Human DHCR7 (7-Dehydrocholesterol Reductase) ELISA Kit | ELK4044 | ELK Biotechnology
Alternative Names: DHCR-7; SLOS; D7SR; Smith-Lemli-Opitz Syndrome; Putative sterol reductase SR-2; Sterol Delta (7) -reductase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the OD of the samples to the standard curve.
