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Product Description
Human DNAJC13 (DnaJ/HSP40 Homolog Subfamily C, Member 13) ELISA Kit | ELK7459 | ELK Biotechnology
Alternative Names: RME8; Required For Receptor-Mediated Endocytosis 8
Species: Human
Assay Type: Sandwich
Sensitivity: 0.33 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DnaJ/HSP40 Homolog Subfamily C, Member 13 (DNAJC13) in the samples is then determined by comparing the OD of the samples to the standard curve.
