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Product Description
Human EAAT5 (Excitatory Amino Acid Transporter 5) ELISA Kit | ELK4414 | ELK Biotechnology
Alternative Names: SLC1A7; AAAT; Solute Carrier Family 1 Member 7, Glutamate Transporter; Retinal glutamate transporter
Species: Human
Assay Type: Sandwich
Sensitivity: 0.122 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 5 (EAAT5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 5 (EAAT5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 5 (EAAT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 5 (EAAT5) in the samples is then determined by comparing the OD of the samples to the standard curve.
