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Product Description
Human EPCA2 (Early Prostate Cancer Antigen 2) ELISA Kit | ELK9102 | ELK Biotechnology
Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin (Protein C) 2, Soluble (Opsonic Defect)
Species: Human
Assay Type: Sandwich
Sensitivity: 2.45 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal Transduction; Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Prostate Cancer Antigen 2 (EPCA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Early Prostate Cancer Antigen 2 (EPCA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Prostate Cancer Antigen 2 (EPCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Early Prostate Cancer Antigen 2 (EPCA2) in the samples is then determined by comparing the OD of the samples to the standard curve.
