Product Description
Human GSTm3 (Glutathione S Transferase Mu 3, Brain) ELISA Kit | ELK5065 | ELK Biotechnology
Alternative Names: GST-M3; GST5; GSTB; GSTM3-3; GTM3
Species: Human
Assay Type: Sandwich
Sensitivity: 1.15 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 3, Brain (GSTm3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Mu 3, Brain (GSTm3) in the samples is then determined by comparing the OD of the samples to the standard curve.