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Product Description
Human hs-Hepc25 (high sensitivity Hepcidin 25) ELISA Kit | ELK9112 | ELK Biotechnology
Alternative Names: Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;
Species: Human
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Lenght: 3.5h
Research Area: Cardiovascular; Signal Transduction; Cardiovascular; Metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to high sensitivity Hepcidin 25 (hs-Hepc25) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to high sensitivity Hepcidin 25 (hs-Hepc25) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain high sensitivity Hepcidin 25 (hs-Hepc25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of high sensitivity Hepcidin 25 (hs-Hepc25) in the samples is then determined by comparing the OD of the samples to the standard curve.
