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Product Description
Human HSPA2 (Heat Shock 70kDa Protein 2) ELISA Kit | ELK4532 | ELK Biotechnology
Alternative Names: HSP-A2; Heat shock-related 70 kDa protein 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.69 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 2 (HSPA2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 2 (HSPA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 2 (HSPA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 2 (HSPA2) in the samples is then determined by comparing the OD of the samples to the standard curve.
