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Product Description
Human HSPb3 (Heat Shock Protein Beta 3) ELISA Kit | ELK4539 | ELK Biotechnology
Alternative Names: HSP-B3; HSPL27; HSP27; HSP 17; Heat Shock 27kDa Protein 3; Heat shock 17 kDa protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.29 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 3 (HSPb3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein Beta 3 (HSPb3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 3 (HSPb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 3 (HSPb3) in the samples is then determined by comparing the OD of the samples to the standard curve.
