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Product Description
Human IgG1 (Immunoglobulin G1) ELISA Kit | ELK5154 | ELK Biotechnology
Alternative Names: IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
Species: Human
Assay Type: Sandwich
Sensitivity: 2.71 ug/mL
Standard: 400 ug/mL
Detection Range: 6.25-400 ug/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G1 (IgG1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G1 (IgG1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G1 (IgG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G1 (IgG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
