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Product Description
Human IS (serum indoxyl sulfate) ELISA Kit | ELK9248 | ELK Biotechnology
Alternative Names: IS; indoxyl sulfate; serum indoxyl sulfate
Species: Human
Assay Type: Sandwich
Sensitivity: 50 ng/mL
Standard: 2000 ng/mL
Detection Range: 31.25-2000 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Kidney diseases
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to serum indoxyl sulfate (IS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to serum indoxyl sulfate (IS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain serum indoxyl sulfate (IS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of serum indoxyl sulfate (IS) in the samples is then determined by comparing the OD of the samples to the standard curve.
