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Product Description
Human KRT81 (Keratin 81) ELISA Kit | ELK6557 | ELK Biotechnology
Alternative Names: KRTHB1; Hb-1; K81; MLN137; Keratin, Hair, Basic 1; Hard Keratin Type II 1; Keratin, type II cuticular Hb1; Metastatic lymph node 137 gene protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 81 (KRT81) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 81 (KRT81) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 81 (KRT81), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 81 (KRT81) in the samples is then determined by comparing the OD of the samples to the standard curve.
